Fisetin Inhibits Hyperglycemia-Induced Proinflammatory Cytokine Production by Epigenetic Mechanisms

Figure 6

Effect of fisetin on chromatin event at the promoter of inflammatory gene. ChIP assays were performed using MAGnify ChIP according to the manufacturer’s instructions. Immunoprecipitations were performed overnight at 4°C with 5 μg of p300 antibody. DNA was subjected to PCR. ChIP assays showed the recruitment of p300 to the TNF-α promoters. Results of 1 typical experiment of 3 are shown. Values from ChIP with anti-p300 antibody represent the fold difference relative to those from IgG control antibody. ^††𝑃<0.01 compared to NG; *𝑃<0.05; **𝑃<0.01 compared to HG. Results are shown as mean ± SD for 3 different experiments.