652059.fig.004a
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652059.fig.004b
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652059.fig.004c
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652059.fig.004d
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652059.fig.004e
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652059.fig.004f
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652059.fig.004g
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652059.fig.004h
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652059.fig.004i
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Figure 4: Effects of DEX and PAN on the distribution and protein expression of TRPC6 at different time points (fluorescencemicroscope ×400). Note: (a, d, g) were the control group, TRPC6 was linear and evenly distributed in the podocytes membrane surface, there are some distribution in the cytoplasm; (b) PAN-inducted 8 h; (e) PAN-inducted 24 h, TRPC6 was not continuity distribution along the cell membrane, increased in the cytoplasm; (h) after PAN-inducted 48 h, TRPC6 distribution increased in the cell membrane, part of the cell membrane lost, gathered into a granular, widely distributed in the cytoplasm; (c) PAN-inducted + DEX-treated 8 h; (f) PAN-inducted + DEX-treated 24 h; (i) PAN-inducted + DEX-treated 48 h, TRPC6 was more homogeneous distribution in the membrane at each time point.