Gallic Acid Attenuates Platelet Activation and Platelet-Leukocyte Aggregation: Involving Pathways of Akt and GSK3β
Figure 3
Inhibitory effects of gallic acid on CD62P expression of platelets stimulated with ADP or U46619. Platelets were pretreated with gallic acid (50 μM, 100 μM, and 500 μM) and then stimulated with (a) ADP (5 μM) or (b) U46619 (5 μM). Surface expression of CD62P on platelets was quantified by flow cytometry. The percentage of platelets positive for CD62P expression (open bars) and the corresponding mean fluorescence intensity (MFI) (filled bars) of the positive platelets were determined. The results were presented as means ± SEM, . *,** versus control samples stimulated by ADP or U46619. GA: gallic acid.
