Research Article

Fractionation of an Extract of Pluchea odorata Separates a Property Indicative for the Induction of Cell Plasticity from One That Inhibits a Neoplastic Phenotype

Figure 5

(a) Thin layer chromatography (TLC) of F4.6.3 (left panel) and F4.3.7 (right panel); mobile phase: TLC system 2; detection: UV254. Analysis of (b) cell cycle and checkpoint regulators, (c) apoptosis-related proteins, (d) oncogenes, (e) proteins required for mobility; 1 × 106 HL60 cells/mL were incubated with 10 μg/mL F4.6.3 and F4.3.7, respectively, and harvested after 0.5, 2, 4, 8, and 24 h of treatment. Cells were lysed and obtained proteins samples applied to SDS-PAGE. Western blot analysis was performed with the indicated antibodies. Equal sample loading was confirmed by Ponceau S staining β-actin or β-tubulin analysis.
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