Research Article

Inhibition of Cell Growth and Induction of Apoptosis by Antrodia camphorata in HER-2/neu-Overexpressing Breast Cancer Cells through the Induction of ROS, Depletion of HER-2/neu, and Disruption of the PI3K/Akt Signaling Pathway

Figure 5

AC altered cell-cycle regulatory proteins in HER-2/neu-overexpressing breast cancer cells. (a) MDA-MB-453 and (b) BT-474 cells were treated with or without AC (40–240 μg/mL) for 24 h. Cyclin D1, cyclin E, p21CIP, p27KIP, Cdk1, Cdk2, Cdk4, and β-actin protein levels in MDA-MB-453 cells and cyclin D1, p21CIP, and β-actin protein levels in BT-474 cells were analyzed by immunoblotting. The proteins (50 μg) in each sample were resolved by 8–15% SDS-PAGE. Relative changes in protein bands were measured by densitometric analysis in which the control was 1.0-fold, as shown immediately below the gel data. The photomicrographs shown in this figure are from one representative experiment that was performed in triplicate with similar results.
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