Inhibition of Cell Growth and Induction of Apoptosis by Antrodia camphorata in HER-2/neu-Overexpressing Breast Cancer Cells through the Induction of ROS, Depletion of HER-2/neu, and Disruption of the PI3K/Akt Signaling Pathway
Figure 6
AC induced apoptosis in HER-2/neu-overexpressing breast cancer cells. (a) TUNEL assay of MDA-MB-453 and BT-474 cells exposed to AC (40–240 μg/mL for 24 h). The average number of apoptosis-positive cells in microscopic fields (magnification ×400) from three separate samples. (b) Western Blot analysis of apoptosis-related proteins in breast cancer cells exposed to AC (40–240 μg/mL for 24 h). The effects of AC on the protein levels of procaspase-3 and -9, PARP, Bcl-2, Bax, and mitochondrial and cytosolic cytochrome c in MDA-MB-453 cells; (c) procaspase-3 and -9, PARP, Bcl-2, and Bax in BT-474 cells. The proteins (50 μg) in each sample were resolved by 8–15% SDS-PAGE with β-actin as a control. Relative changes in protein bands were measured by densitometric analysis in which the control was 1.0-fold, as shown immediately below the gel data. The photomicrographs shown here are from one representative experiment repeated two times with similar results.
