705167.fig.003a
(a)
705167.fig.003b
(b)
Figure 3: Effects of AKH extract on TARC and histamine production in HaCaT cells and HMC-1 cells, respectively. The production of TARC (a) was measured in culture medium of cells treated with AKH extract (2, 5, or 10 μg/mL) and then costimulated with TNF-α and IFN-γ (each 10 ng/mL, TI) for 24 h. Silymarin (20 and 50 μg/mL) was used as a positive control drug. The production of histamine (b) was measured in culture medium of cells treated with AKH extract (10 and 20 μg/mL) and then costimulated with PMA (50 nM) and A23187 (1 μM) for 24 h. Each bar represents the mean of three independent experiments. ##P < 0.01 versus vehicle control group; *,**P < 0.05 and P < 0.01 versus TI-treated cells, respectively.