Rats Born to Mothers Treated with Dexamethasone 15 cH Present Changes in Modulation of Inflammatory Process

Table 4

Number of inflammatory cells in the subcutaneous tissue of the footpaw inoculated with 1% kappa carrageenan. PMN cells were counted using HE stained slides. Immuno-histochemistry was used to identify different stages of phagocyte maturation (CD 163) and the number of mononuclear phagocytes per field (MAC 387). Adhesion molecules CD18 (beta integrin) and CD54 (ICAM 1) were marked in leukocytes and endothelial cells. The intensity of CD54 marked cells was measured by pre-defined scores measured by three independent observers. The proportion of degranulated mast cells (N/200 total counted cells) is represented by percentage. The values are represented by mean (𝜒) and standard deviation (SD). ^*Student “t” test, 𝑃≤0.01 in relation to control (CONT); ^#𝜒², 𝑃=0.001 in relation to negative/positive ratio of control group (CONT).

	Dexamethasone 15 cH	Control
Eosin/hematoxylin (PMN)	𝜒 = 0.54; SD=2.114	𝜒 = 0.33; SD=1.124
MAC 387 + (Macrophages)	𝜒 = 0.58^*; SD=0.923	𝜒 = 1.40; SD=1.725
CD163 + (negative monocytes)	𝜒 = 4.28^#; SD=5.386	𝜒 = 4.99; SD=5.741
CD163 + Membrane-marked monocytes	𝜒 = 0.790^*; SD=1.664	𝜒 = 0.321; SD=0.919
CD163 + cytoplasm marked monocytes	𝜒 = 7.00; SD=5.399	𝜒 = 6.76; SD=6.550
CD163 + cytoplasm marked macrophages	𝜒 = 0.533333; SD=0.787863	𝜒 = 0.491525; SD=0.90342
CD 18 + (positive mononuclear cells)	𝜒 = 1.55; SD=1.559429	𝜒 = 1.922222; SD=3.638829
CD 18 + (negative mononuclear cells)	𝜒 = 8.944444; SD=3.724686	𝜒 = 10.21111; SD=4.384586
CD 18 + (positive total leukocytes)	𝜒 = 18.74^*; SD=8.15	𝜒 = 15.41; SD=7.68
CD 18 + (negative total leukocytes)	𝜒 = 0.75; SD=1.06	𝜒 = 0.55; SD=0.99
CD 54 + (scores)	𝜒 = 2.33; SD=1.057	𝜒 = 2.41; SD=1.057
Degranulated mast cells (n/200 counted cells)	9.5/200 (4.75%)^#	5.9/200 (2.95%)