Antileukemic Potential of Momordica charantia Seed Extracts on Human Myeloid Leukemic HL60 Cells
Figure 1
(a) Effects of different fractions of M. charantia seeds on HL60 cells differentiation. HL60 cells treated with varying concentrations of different fractions were scored for the percentage of NBT positive cells on day 5. Mc-1-(5–10 μg/mL) and Mc-2-(5–20 μg/mL) treated cells showed a significant increase in NBT staining compared to untreated control. (b) Effect of different fractions of M. charantia seeds on HL-60 cell viability. HL60 cells (105 cells/mL) were cultured in 96-well plates and incubated with the indicated concentrations of fractions Mc-1, Mc-2, and Mc-3, for 72 h. The MTT assay was used to assess cell viability. The optical density value of the control was regarded as 100%. Data represent the mean ± SD of three independent experiments. (c) Time dependence of Mc-2 induced HL60 cells differentiation. Control, and Mc-2 (20 μg/mL) or DMSO-(included as a positive control) treated HL60 cells were scored for percentage of differentiation by NBT staining from day 1 to 5. (d) Effect of heat-inactivated Mc-2 (Mc-2H) and S. griseus protease-treated (Sigma Aldrich, USA) Mc-2 (Mc-2P) on HL60 cells differentiation. HL60 cells treated with indicated fractions (20 μg/mL each) were scored for the NBT positive cells on day 5. All the three fractions resulted in significantly increased NBT staining, relative to untreated control. The data in all the panels represent mean ± SD of three independent experiments performed in triplicates. *, **, and *** relative to untreated control.
