Chlorella sorokiniana-Induced Activation and Maturation of Human Monocyte-Derived Dendritic Cells through NF-κB and PI3K/MAPK Pathways
Figure 2
Allogeneic T-cell responses induced by CS-treated DCs. Immature DCs were stimulated with CS (30 μg/mL) or LPS (1 μg/mL) for 48 h. Allogeneic T cells () were cocultured in 96-well U-bottom microplates with CS-treated DCs as described. (a) T-cell proliferation was determined by using an Alamar Blue assay 5 days post coculturing. Experimental groups were compared to corresponding control groups. (b) After 2 days of coculturing, the production of IFN-γ by T cells was analyzed by ELISA. Data were expressed as means ± SEM of triplicates from three independent experiments. * compared to control. N.D: nondetectable.
