Flavonoid Myricetin Modulates 𝐆𝐀𝐁𝐀_𝐀 Receptor Activity through Activation of 𝐂𝐚^𝟐+ Channels and CaMK-II Pathway

Figure 5

Myricetin increases CaMK-II phosphorylation in primary culture of rat hypothalamic neurons and PC-12 cell line. (a) Myricetin increases CaMK-II phosphorylation in hypothalamus neurons. DMSO: incubated with 0.5% DMSO for 10 min; Myricetin: incubated with 50 μM myricetin for 10 min. Arrows indicate neuronal cell bodies (Green: phosphorylated CaMKII; red: beta-tubulin). (b) Myricetin increases phosphorylated CaMK-II in PC-12 cell line. Control, blank control; DMSO, 0.1% DMSO in PBS; MYR: myricetin, 50 μM in PBS with 0.05% DMSO; FSK: forskolin, 10 μM in PBS with 0.1% DMSO.

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