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Evidence-Based Complementary and Alternative Medicine
Volume 2012 (2012), Article ID 781280, 10 pages
http://dx.doi.org/10.1155/2012/781280
Research Article

The Dual Antimelanogenic and Antioxidant Activities of the Essential Oil Extracted from the Leaves of Acorus macrospadiceus (Yamamoto) F. N. Wei et Y. K. Li

1Department of Medical Laboratory Science and Biotechnology, China Medical University, No. 91 Hsueh-Shih Road, Taichung 40402, Taiwan
2Department of Hair Styling and Design, Hung Kuang University, No. 34, Chung-Chie Rd., Shalu, Taichung City 43302, Taiwan
3Department of Applied Cosmetology & Master Program of Cosmetic Science, Hung Kuang University, No. 34, Chung-Chie Rd., Shalu, Taichung City 43302, Taiwan
4Crop production Section, General of Agriculture Bureau of Taichung City, Taichung, No. 89, Sec 2, Taichung Port Road, Xitun District, Taichung City 40701, Taiwan

Received 18 July 2012; Accepted 6 September 2012

Academic Editor: Edourad Hnawia

Copyright © 2012 Huey-Chun Huang et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The antimelanogenic and antioxidant activities of the essential oil extracted from the leaves of Acorus macrospadiceus (Yamamoto) F. N. Wei et Y. K. Li have never been explored. The essential oil effectively inhibited mushroom tyrosinase activity (EC50 = 1.57 mg/mL) and B16F10 tyrosinase activity (IC50 = 1.01 mg/mL), decreased the melanin content (EC50 = 1.04 mg/mL), and depleted the cellular level of the reactive oxygen species (ROS) (EC50 = 1.87 mg/mL). The essential oil effectively scavenged 2,2-diphenyl-1-picryl-hydrazyl (DPPH) (EC50 = 0.121 mg/mL) and 2,2′-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) ABTS+ radicals (EC50 = 0.122 mg/mL). It also exhibited an apparent reducing power (EC50 = 0.021 mg/mL) and metal-ion chelating activity (EC50 = 0.029 mg/mL). The chemical constituents of the essential oil are ethers (55.73%), ketones (19.57%), monoterpenes (7.82%), alcohols (3.85%), esters (3.77%), sesquiterpenes (3.72%), and aromatic compounds (2.85%). The results confirm that A. macrospadiceus essential oil is a natural antioxidant and inhibitor of melanogenesis.