Immunomodulatory Effect of Rhaphidophora korthalsii on Natural Killer Cell Cytotoxicity
Table 1
Mice splenocytes harvested after day 5 or day 15 of treatment were allowed to proliferate with or without the treatment of R. korthalsii (25 g/mL) or mouse rIL-2 (50 U/mL). The rate of cell viability was evaluated using MTT cell viability assay*.
Untreated
R. korthalsii (25 g/mL)
Mouse rIL-2 (50 U/mL)
Splenocytes (%)
Bone marrow (%)
Splenocytes (%)
Bone marrow (%)
Splenocytes (%)
Bone marrow (%)
Control
5 day
100
100
172 ± 1.3
152 ± 2.6
186 ± 2.1
160 ± 3.2
15 day
100
100
168 ± 2.1
154 ± 2.3
179 ± 3.4
159 ± 2.1
R. korthalsii (25 g/mice)
5 day
104 ± 1.6
106 ± 1.3
178 ± 2.7
147 ± 1.4
184 ± 1.6
158 ± 2.7
15 day
102 ± 1.1
107 ± 2.5
174 ± 1.5
149 ± 1.9
187 ± 1.8
162 ± 2.8
R. korthalsii (200 g/mice)
5 day
113 ± 1.2
107 ± 2.4
184 ± 3.2
159 ± 2.7
192 ± 3.4
163 ± 1.8
15 day
118 ± 1.6
110 ± 2.5
180 ± 2.7
151 ± 1.7
195 ± 2.3
171 ± 1.9
R. korthalsii (350 g/mice)
5 day
154 ± 1.8*
161 ± 2.8*
247 ± 2.9*
201 ± 4.8*
253 ± 4.1*
214 ± 3.7*
15 day
151 ± 2.1
168 ± 3.4
241 ± 3.2
213 ± 3.6
258 ± 4.7
209 ± 4.2
R. korthalsii (700 g/mice)
5 day
146 ± 2.3*
137 ± 2.4*
225 ± 3.4*
187 ± 3.1*
241 ± 3.5*
193 ± 3.1*
15 day
142 ± 1.5
141 ± 2.3
233 ± 4.6
192 ± 4.2
245 ± 3.7
204 ± 2.5
Mice rIL-2 (50 U/mice)
5 day
143 ± 2.1*
172 ± 4.3*
259 ± 4.2*
211 ± 4.9*
267 ± 3.3*
219 ± 1.8*
15 day
124 ± 2.7
121 ± 2.6
197 ± 3.5
184 ± 5.1
207 ± 3.5
192 ± 1.9
*Note: the values are the means ± SE of three experiments. The differences between the control group and treated group were determined by one-way ANOVA (*).
