Fisetin Inhibits Osteoclast Differentiation via Downregulation of p38 and c-Fos-NFATc1 Signaling Pathways
Figure 2
Fisetin inhibits RANKL-induced phosphorylation of p38 and expression of c-Fos and NFATc1. (a) BMMs were pretreated with vehicle or fisetin (5 μM) for 1 h prior to RANKL stimulation (5 ng/mL) at indicated time periods. Then, protein expression levels were evaluated by western blot analysis. Actin was used as the internal control. (b) BMMs were stimulated with RANKL (5 ng/mL) and M-CSF (30 ng/mL) in the presence or absence of fisetin (5 μM) for the indicated times. Then, total RNA was isolated from cells using TRIzol reagent, and mRNA expression levels were evaluated by real-time PCR. * (versus “the vehicle control”). (c) Effects of fisetin on protein expression levels of c-Fos and NFATc1 were evaluated by western blot analysis. Actin was used as the internal control.
