Terpinen-4-ol Induces Apoptosis in Human Nonsmall Cell Lung Cancer In Vitro and In Vivo
Figure 3
Effects of terpinen-4-ol on caspase activation in A549 and CL1-0 cells. (a) Cells were treated with the indicated concentrations of terpinen-4-ol for 24 hours. Total cell lysates were prepared, resolved by SDS-PAGE, and immunoblotted with the indicated antibodies to detect the cleaved forms of caspase-8, caspase-9, caspase-3, and PARP. Data are representative of three independent experiments with similar results. (b) Cells were treated with terpinen-4-ol and/or the indicated caspase inhibitor for 24 hours, and cell viability was determined using the MTT assay. Data are mean ± SD of three independent experiments.
