Research Article

Terpinen-4-ol Induces Apoptosis in Human Nonsmall Cell Lung Cancer In Vitro and In Vivo

Figure 4

Effects of terpinen-4-ol on mitochondrial membrane potential and cytochrome c release in A549 and CL1-0 cells. (a) The cells were stained with JC-1 fluorescence dye and the change in mitochondrial membrane potential was examined by flow cytometry. Data are representative of three independent experiments with similar results. (b) Cytosolic lysates were prepared and subjected to SDS-PAGE followed by Western blotting with anticytochrome c antibody. Data are representative of three independent experiments showing similar results.
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