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Evidence-Based Complementary and Alternative Medicine
Volume 2012 (2012), Article ID 819632, 12 pages
http://dx.doi.org/10.1155/2012/819632
Research Article

Antimigratory Effects of the Methanol Extract from Momordica charantia on Human Lung Adenocarcinoma CL1 Cells

1Department of Life Sciences, Tzu-Chi University, Hualien, Taiwan
2Institute of Medical Sciences, Tzu-Chi University, Hualien, Taiwan
3Department of Anatomy, Tzu-Chi University, Hualien, Taiwan
4Hualien District Agricultural Research and Extension Station, Hualien, Taiwan
5National Institute of Cancer Research, National Health Research Institutes, Zhunan, Taiwan

Received 24 June 2012; Revised 26 October 2012; Accepted 12 November 2012

Academic Editor: Jen-Hwey Chiu

Copyright © 2012 Hsue-Yin Hsu et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Momordica charantia has been found to exhibit anticancer activity, in addition to its well-known therapeutic functions. We have demonstrated that the leaf extract of Momordica charantia (MCME) induces apoptosis in several human cancer cells through caspase- and mitochondria-dependent pathways. In this study, a different susceptibility to MCME was found in human lung adenocarcinoma CL1 cells with different metastatic ability, leading to the significant difference of cell viability and invasiveness between MCME-treated CL1-0 and CL1-5 cells. MCME was found to upregulate the expression of Wnt-2 and affect the migratory and invasive ability of CL1 cells through suppressed MMP-2 and MMP-9 enzymatic activities. We proposed that MCME mediates inhibition against migration of CL1 cells by reducing the expression and activation of Src and FAK to decrease the expression of downstream Akt, β-catenin, and MMPs.