Topical Application of Chrysanthemum indicum L. Attenuates the Development of Atopic Dermatitis-Like Skin Lesions by Suppressing Serum IgE Levels, IFN-γ, and IL-4 in Nc/Nga Mice
Figure 4
IL-4, IL-13, and IFN-γ expression in the dorsal skin. AD induced in Nc/Nga mice by topical application of 2,4-dinitrochlorobenzene (DNCB) in the dorsal skin, and a right ear was topically treated with 1,3-butylen glycol (BG; control), 5% Chrysanthemum indicum L. (CIL-Low), 30% Chrysanthemum indicum L. (CIL-High), and 0.1% hydrocortisone (HC) on the lesions for 5 weeks. Normal controls did not have DCNB-induced AD. After 5 weeks of treatments, total RNA was extracted from the dorsal skin, and cDNA was generated. The mRNA expression of IL-4, IL-13, and INF-γ was measured by realtime PCR, and their relative expression was standardized according to respective β-actin mRNA levels. CIL-High significantly decreased the expression of IL-4, IL-13, and INF-γ in the lesion of the dorsal skin in comparison to the control and exhibited a statistically similar decrease as HC. Each value represents the mean ± SD of 5 mice in each group. *Significantly different among the different treatments in Nc/Nga mice at . a, b, c Means on the bars with different superscripts were significantly different by Tukey test at . †Significantly different between control (BG) and normal control at .
