Research Article
The Antiparkinsonian and Antidyskinetic Mechanisms of Mucuna pruriens in the MPTP-Treated Nonhuman Primate
Table 2
Dosing regimen for non-human primates. A block design was devised for each testing session. Each behavioral testing cohort was varied to maintain blind and to prevent the behavioral rater from guessing the treatments. Each such block design was repeated twice for each experiment and videos are independently rated. Representative animals were videotaped continuously in the room 24 × 5 × 365 days. The postdrug treatment videos were culled from these videos by the person who administered the drug who was unblinded. These culled video segments were used for the scoring along with the rater executed direct observational scoring of mUPDRS and AIMS. These culled segments began as soon as the person administering the drug confirmed successful consumption of the drug and lasted 8 hours from that time. The notion of the average time of 75 minutes refers to the average time at which behavioral ratings using mUPDRS and AIMS were scored for the study. The validity of this time frame for the detection of optimal effects of LD and LD/DDCI oral treatments has been published previously. To make meaningful valid comparisons MPEP and MPWE treatments were also performed at the same time schedule. The remainder of the video was rated, but it does not have the observer interaction and it only shows routine animal activity in its home cage. As expected, LD and LD/DDCI treatments had behavioral benefits that lasted 180 minutes and then ameliorated. Effects of MPEP and MPWE lasted longer and appeared to dissipate only after 6 hours.
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