Dentatin Induces Apoptosis in Prostate Cancer Cells via Bcl-2, Bcl-xL, Survivin Downregulation, Caspase-9, -3/7 Activation, and NF-κB Inhibition
Figure 10
Dentatin inhibits TNF-α-induced NF-κB nuclear translocation in prostate cancer cells. (a) Representative images of NF-κB localization in the cellular compartment of PC-3 and LNCaP cells without stimulation (TNF-α −), with 10 ng/mL TNF-α stimulation alone (TNF-α +) or treated with 15 μM of dentatin after 10 ng/mL TNF-α stimulation. Cells were stained with Hoechst 33342 for nucleus (blue). NF-κB (green) was stained as described in Section 2. 20x magnification. (b) Histogram showing quantitative fluorescence intensity analysis of NF-κB in the nucleus of PC-3 and LNCaP without stimulation (TNF-α −), with 10 ng/mL TNF-α stimulation alone (TNF-α +) or treated with various concentrations of dentatin after 10 ng/mL TNF-α stimulation. Dentatin treatment caused a dose-response decreased of TNF-α-induced NF-κB nuclear translocation in PC-3 and LNCaP cells. Data were shown as mean ± SD. Significant differences were determined by comparing samples to the TNF-α stimulated cells (TNF-α +).
