About this Journal Submit a Manuscript Table of Contents
Evidence-Based Complementary and Alternative Medicine
Volume 2012 (2012), Article ID 872406, 6 pages
http://dx.doi.org/10.1155/2012/872406
Research Article

Combined Effects of Eurycoma longifolia and Testosterone on Androgen-Deficient Osteoporosis in a Male Rat Model

1Department of Biomedical Sciences, Faculty of Health Sciences, National University of Malaysia (Universiti Kebangsaan Malaysia), 50300 Kuala Lumpur, Malaysia
2Department of Pharmacology, Faculty of Medicine, National University of Malaysia (Universiti Kebangsaan Malaysia), 50300 Kuala Lumpur, Malaysia

Received 2 May 2012; Revised 26 June 2012; Accepted 16 July 2012

Academic Editor: Norazlina Mohamed

Copyright © 2012 Halimatun Saadiah Abdul Razak et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Androgen-deficient osteoporosis in men is treated with testosterone therapy, which is associated with side effects. Eurycoma longifolia (EL) is known to possess androgenic properties and has been reported to protect bone from androgen-deficient osteoporosis in experimental animal models. The present study aimed to determine the effectiveness of combination therapy of EL and testosterone (T) in treating androgen-deficient osteoporosis. Forty male Sprague-Dawley rats were divided into: sham-operated (SHAM), orchidectomized-control (ORX), orchidectomized with testosterone (ORX + T), orchidectomized with EL (ORX + EL), and orchidectomized with combined T and EL therapy (ORX + T + EL). EL was administered via oral gavages daily at the dose of 15 mg/kg. T was injected intramuscularly at 8 mg/kg and 4 mg/kg for the ORX + T and ORX + T + EL groups, respectively. Following 6 weeks of treatment, the osteocalcin levels of ORX + T and ORX + T + EL groups were significantly lower than the SHAM group ( . The posttreatment CTX levels of ORX + T and ORX + T + EL groups were significantly lower than their pretreatment levels ( . Biomechanically, the strain parameter of the ORX + T + EL group was significantly higher than the ORX group ( . Thus, the combination therapy of EL and low-dose T has potential for treatment of androgen-deficient osteoporosis. The lower T dose is beneficial in reducing the sideeffects of testosterone therapy.