Active Hydrophilic Components of the Medicinal Herb Salvia miltiorrhiza (Danshen) Potently Inhibit Organic Anion Transporters 1 (Slc22a6) and 3 (Slc22a8)
Figure 2
Inhibition profile of mOat1 and mOat3. (a) Inhibition of mOat1-mediated uptake of [3H]PAH (5 μM) by LSA, PCA, RMA, SAA, SAB, TSL, and probenecid (1000 μM) was measured in the CHO-mOat1 cells (10 min). Background PAH accumulation was measured in CHO-FRT cells in the absence of inhibitor and is shown to provide a clear gauge of the low background in the experimental system. (b) Inhibition of mOat3-mediated uptake of [3H]ES (1 μM) by LSA, PCA, RMA, SAA, SAB, TSL, and probenecid (1000 μM) was measured in the CHO-mOat3 cells (10 min). Background ES accumulation was measured in CHO-FRT cells in the absence of inhibitor and is shown to provide a clear gauge of the low background in the experimental system. Values are mean ± S.D. of triplicate values. ***denotes as determined by one-way ANOVA followed by Dunnett’s t-test.
