Figure 5: determination for LSA, RMA, and SAA in the CHO-mOat3 cell line. One minute uptake of [3H]ES (1 μM) in CHO-mOat3 cells was measured in the presence of increasing concentrations (10−7 to 4 × 10−4 M) of LSA, RMA, and SAA. Data were corrected for nonspecific background measured in the CHO-FRT cells prior to kinetic analysis. values were determined with non-linear regression and the “one-site competition” model using GraphPad Prism software. Experiments were repeated three times in triplicate with the mean ± S.E.M. reported in Table 2. Graphs shown are from representative experiments with values plotted as mean S.D. ( ).