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Evidence-Based Complementary and Alternative Medicine
Volume 2012 (2012), Article ID 908601, 12 pages
Herbal Compound “Songyou Yin” Renders Hepatocellular Carcinoma Sensitive to Oxaliplatin through Inhibition of Stemness
1Liver Cancer Institute, Zhongshan Hospital, Fudan University and Key Laboratory of Carcinogenesis and Cancer Invasion, Ministry of Education, 180 Fenglin Road, Shanghai 200032, China
2Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, China
Received 12 October 2012; Accepted 12 November 2012
Academic Editor: Hui-Fen Liao
Copyright © 2012 Qing-An Jia et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Additional file 1: The expression of CSC-related markers in the cell lines HCCLM3, MHCC97H, HepG2, SMMC7721, Hep3B, and Huh7 was examined by flow cytometry and immunofluorescence.
Additional file 2: Tumor cells that were pretreated with SYY for 4 weeks showed reduced expression of CSC-related markers compared with parental cell lines by flow cytometric analysis.
Additional file 3: The transwell assay demonstrated that MHCC97H-SYY and Hep3B-SYY cells migrated through the basement membrane less efficiently than the parental cell lines MHCC97H and Hep3B.
Additional file 4: Colony formation assay showed that MHCC97H-SYY and Hep3B-SYY cells had a significantly lower proliferation rate and colony forming ability than the corresponding parental cells.
Additional file 5: MHCC97H-SYY cell lines showed diminished subcutaneous tumor growth capacity and decreased pulmonary metastasis compared with parental cells in the nude mouse models.