Effects of Extracts from Trifolium medium L. and Trifolium pratense L. on Development of Estrogen Deficiency-Induced Osteoporosis in Rats

Table 6

Effects of Trifolium medium L. (TM) and Trifolium pratense L. (TP) extracts on the serum bone turnover markers in ovariectomized (OVX) rats.

Parameters	Sham-; operated rats	OVX rats
		Control	TM10	TM20	TP10	TP20	Estradiol
Osteocalcin (ng/mL)	238.30; ± 27.70	325.47; ± 26.72	327.36; ± 28.13	344.84; ± 25.61	344.84; ± 38.73	332.50; ± 39.91	254.33; ± 17.13
RatLaps (ng/mL)	20.88; ± 1.82	30.89; ± 1.44**	30.79; ± 1.99**	30.74; ± 1.93**	32.99; ± 1.78***	28.59; ± 2.09*	23.39; ± 2.80^●

The lyophilized extracts from TM or TP at doses corresponding to 10 and 20 mg/kg of isoflavone aglycones daily (TM10, TP10 and TM20, TP20, resp.), or estradiol (0.2 mg/kg daily), were administered orally to OVX rats for 4 weeks. Results are presented as the mean ± SEM (𝑛=9-10). One-way ANOVA followed by Duncan’s test was used for evaluation of the significance of the results. *𝑃<0.05, **𝑃<0.01, ***𝑃<0.001—significantly different from sham-operated control rats. ^●𝑃<0.05—significantly different from OVX control rats.