Resveratrol Produces Neurotrophic Effects on Cultured Dopaminergic Neurons through Prompting Astroglial BDNF and GDNF Release
Figure 2
Astroglia were responsible for resveratrol-mediated neurotrophic effects. Three types of cultures including neuron-enriched, neuron-astroglia, and neuron-microglia reconstituted cultures were treated with resveratrol (100 M) on the first day. The [] DA uptake assessment was performed 7 days after resveratrol administration (a). The astroglia-conditioned medium (ACM) was prepared from primary astroglia cultures treated with 100 M resveratrol (ACM-resveratrol) or control (ACM) for 48 h. ACM and ACM-resveratrol were collected and added to the neuron-enriched cultures and incubated for 7 days. The dompaminergic neuronal function was evaluated by the [] DA uptake assay (b). Results were expressed as a percentage of the control cultures and were the mean ± SEM from three independent experiments performed in triplicate. compared with the control cultures; compared with the ACM cultures.
