Cucurbitacin E Induces G2/M Phase Arrest through STAT3/p53/p21 Signaling and Provokes Apoptosis via Fas/CD95 and Mitochondria-Dependent Pathways in Human Bladder Cancer T24 Cells
Figure 5
The effects of a general caspase inhibitor (Z-VAD-FMK) on cucurbitacin E-induced cell viability and caspase cascades protein levels on T24 cells. Cells were pretreated with 10 μM Z-VAD-FMK and then exposed to 1000 nM cucurbitacin E for 48 h. Cells were harvested and determined for percentage of viable cells (a) and for evaluating the protein levels of caspase-8, -9, and -3 (b) as described in Materials and Methods. Columns: mean (); bars: SD. a, , is significantly different compared with untreated control; b, , shows significant difference compared with 1000 nM cucurbitacin E treatment group by one-way ANOVA followed by Bonferroni’s multiple comparison test.
