Cucurbitacin E Induces G2/M Phase Arrest through STAT3/p53/p21 Signaling and Provokes Apoptosis via Fas/CD95 and Mitochondria-Dependent Pathways in Human Bladder Cancer T24 Cells
Figure 6
The effects of cucurbitacin E on mitochondrial membrane potential () in T24 cells. Cells at a density of cells/well placed in 12-well plates were treated with 1000 nM cucurbitacin E for 0, 4, 8, 12, 24, and 48 h and then harvested for measuring the level of as described in Materials and Methods. (a) The representative of profile of , and (b) the levels of quantified form BD CellQuest Pro software. Each experiment was done with triple sets: a, , shows significant difference compared with 0 h treatment; b, c, and d, , are significantly different compared to cucurbitacin E treatment for 4, 8, and 12 h, respectively, by one-way ANOVA followed by Bonferroni’s multiple comparison test.
