Astragaloside IV Downregulates β-Catenin in Rat Keratinocytes to Counter LiCl-Induced Inhibition of Proliferation and Migration
Figure 3
BrdU staining of cells treated with LiCl in the absence of presence of AS-IV. (a) LiCl treatment produced a concentration-dependent decrease in BrdU-positive cells compared to control cells. (b) Histograms represent the mean ± SD of the percent of BrdU-positive cells treated with different concentrations of LiCl. (c) Changes in β-catenin protein expression in response to LiCl. (d, e, f) Comparison of the effects of LiCl ± AS-IV on cell proliferation, by detection of BrdU incorporation and β-catenin protein expression. AS-IV resolved LiCl-induced inhibition of cell proliferation and downregulation of β-catenin. * . Expression of K14 and GAPDH were detected as protein loading controls Scale bar = 100 μm.
