Astragaloside IV Downregulates β-Catenin in Rat Keratinocytes to Counter LiCl-Induced Inhibition of Proliferation and Migration
Figure 8
AS-IV attenuates LiCl-induced β-tubulin downregulation. Keratinocytes were treated with LiCl (20 mM) and LiCl (20 mM) + AS-IV (80 μg/mL) for 24 h, and β-tubulin was determined by immunofluorescence (a, merged images of K14/β-tubulin/DAPI were shown) and immunoblotting ((c), GAPDH protein expression was used as the protein loading control). (b) Cell numbers of β-tubulin-positive keratinocytes per 100 total cells are presented. AS-IV significantly attenuated LiCl-induced β-tubulin downregulation. *, **. Scale bar = 100 μm.
