Research Article

Pseudolaric Acid B Induces Caspase-Dependent and Caspase-Independent Apoptosis in U87 Glioblastoma Cells

Figure 2

Effect of PLAB on U87 glioblastoma cell viability. (a) U87 cells were treated with indicated concentrations of PLAB and doxorubicin (positive control) for 24 h and cell viability was determined by MTT assay. Data are expressed as Mean ± SEM ( ). Columns not sharing the same superscript letter differ significantly ( ). (b) U87 cells were treated with DMSO or 5 and 10  M PLAB for 24 h. Following treatment, cells were harvested, washed with PBS, and stained with 2 μM calcein AM and 4 μM PI in the dark for 20 min. After washing, cells were resuspended in PBS and samples were analyzed by flow cytometry for the quantification of live and dead cells. (c) Data of figure (b) are expressed as Mean ± SEM ( ). Columns not sharing the same superscript letter differ significantly ( ).
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