Pseudolaric Acid B Induces Caspase-Dependent and Caspase-Independent Apoptosis in U87 Glioblastoma Cells

Figure 7

Effect of PLAB on apoptosis regulators in U87 glioblastoma cells. U87 glioblastoma cells were treated with indicated concentrations of PLAB for 24 h. Following treatment, cells were harvested and total, cytosolic and nuclear proteins were extracted as described in Materials and Methods section. The expression of p53, Bcl-2, Bax, Caspase-3, PARP, cytochrome c, and AIF were analyzed by Western blot analysis.