Salvianolic Acid B Inhibits ERK and p38 MAPK Signaling in TGF-1-Stimulated Human Hepatic Stellate Cell Line (LX-2) via Distinct Pathways
Figure 1
Inhibition of Smad4 in LX-2 transfected with Pol II miR RNAi expression vectors (SRV1, SRV2, SRV3, and SRV4). (a) Smad4 mRNA expression levels detected by real time PCR 72 h after selection with blasticidin. Results were normalized to Smad4 expression in “Negative control” using the method (where Ct is threshold cycle). **Significant difference versus negative control (, ). On the right side of the figure is shown the electrophoresis of PCR product. (b) Smad4 protein expression levels detected after selection with blasticidin, using the Western blot. Blotting with anti-β-actin antibody was conducted as a protein loading control. Quantification of the intensity of bands calibrated to the intensity of total protein bands (means ± SD). **Significant difference versus negative control (, ).
