Salvianolic Acid B Inhibits ERK and p38 MAPK Signaling in TGF-𝜷1-Stimulated Human Hepatic Stellate Cell Line (LX-2) via Distinct Pathways

Table 2

The changes of Col. I in LX-2 cell culture supernatant after inhibition of p38 MAPK and Smad4 signaling.

Group	𝑛	Mean ± S
Control	3	0.567 ± 0.093
Negative control	3	0.469 ± 0.004
SRV4	3	0.351 ± 0.011
TGF	3	1.007 ± 0.017^##
SM4 + TGF	3	0.648 ± 0.012^▲▲
SM4 + TGF + SA-B	3	0.289 ± 0.022
SM4 + TGF + SB	3	0.281 ± 0.036
TGF + SA-B + SB	3	0.386 ± 0.024**
SM4 + TGF + SA-B + SB	3	0.222 ± 0.026^Δ

The levels of Col. I protein were determined by ELISA kit as indicated. Results were expressed as mean ± SE of three independent experiments performed in triplicate. ^##Significant difference versus Control, Negative control, and SRV4 (𝑛=3,𝑃<0.01); ^▲▲Significant difference versus SRV4 and TGF-β1 (𝑛=3,𝑃<0.01); **Significant difference versus SRV4 (𝑛=3, 𝑃<0.01); ^ΔSignificant difference versus SM4 + TGF + SA-B, SM4 + TGF + SB, and TGF + SA-B + SB (𝑛=3, 𝑃<0.05).