Figure 2: Rottlerin did not induce apoptosis in cells. (a) Western blot analysis of the antiapoptotic protein Bcl-2, caspase 9, caspase 3, and PARP after 1–24 h of 20 μM Rottlerin treatment; β-actin was used as loading control. (b) Nuclear staining with Hoechst 33342 after 18 and 24 h treatment; o.m.: original magnification. Representative of three independent experiments.