Figure 5: Rottlerin induced Bcl-2/Beclin-1-independent autophagic death in cells. (a) Western blotting analysis of Beclin-1 expression after 20 μM Rottlerin treatment for 1–24 h. (b) Western blotting analysis of LC3 after 1–10 μM of Rottlerin treatment for 24 h in Bcl-2-silenced and Bcl-2-overexpressing cells. (c) Western blotting analysis of LC3 after 20 μM of Rottlerin treatment for 6 h, in the presence and absence of 5 mM 3-MA. β-actin was used as loading control. Representative of two independent experiments.