Figure 6: Morphological and biochemical features of apoptosis in Rottlerin-treated cells: (a) Cell survival expressed as % of seeded cells (control), evaluated by the SRB assay. (b) Phase-contrast microscopy and (c) Hoechst 33342 staining after 18 and 24 h of 20 μM Rottlerin exposure; o.m.: original magnification. (d) Western blotting analysis of Bcl-2, caspases 9, caspases 3, PARP, and LC3-I-II after 20 μM of Rottlerin treatment for 1–24 h. β-actin was used as a loading control. Representative of three independent experiments.