Selected Extracts of Chinese Herbal Medicines: Their Effect on NF-κB, PPARα and PPARγ and the Respective Bioactive Compounds

Figure 3

Comparative analysis by GC-MS of the DCM-I and -II and EtOAc VLC fractions (1 μL of 4 mg/mL in DCM) from the mother DCM extract of CHMs nos. 1a of A. julibrissin. FAMEs formed from the present FAs after derivatisation by TMSH were detected by GC-MS on a Zebron ZB-5 column (60 m × 0.25 mm, 0.25 μm) (Phenomenex) with a temperature gradient of 120°C-320°C in 40 min and 5 min hold; carrier gas: helium; flow rate: 1.7 mL/min. Ion source: EI 70 eV, 250°C. The following FAs were identified by GC-MS with a relative content (%) in each enriched extract given in the order DCMI; DCMII and EtOAc: (1) palmitic acid (2.9; 37; 23%), (2) linoleic acid (4.5; 1.0; 47%), (3) 9-octadecenoic acid (3.3; 5.1; 4.4%), (4) oleic acid (—; 3.8; 5.8%), (5) octadecanoic acid (3.3; 9.4; 2.2%), (6) docosanoic acid (—; 4.5; 1.7%), and (7) tetracosanoic acid (—; 2.3; 3.0%).