Figure 6: Effects of the Yangjing capsule extract on apoptosis and caspase-3 expression in MLTC-1 cells. The Yangjing capsule extract was added to MLTC-1 cells for 48 h without serum. The cells were collected and analyzed using flow cytometry. The expression of caspase-3 mRNA and protein was detected by real-time RT-PCR and a colorimetric assay. (a) The percentages of viable cells are shown in the lower left quadrant, and the percentages of apoptotic cells are shown in the lower right quadrant. (b) The ratios of the above cells are represented as percentages. (c) The effect of the Yangjing capsule extract on expression of caspase-3 mRNA. (d) The effect of the Yangjing capsule extract on caspase-3 protein activity. The determination of caspase-3 activity was performed using a caspase-3 colorimetric protease assay kit. The results are given as the means ± SD from three independent experiments. Compared with control, , .