Induction of Apoptosis by Luteolin Involving Akt Inactivation in Human 786-O Renal Cell Carcinoma Cells
Figure 2
Effects of luteolin on MAPKs and Akt. 786-O cells were treated with various concentrations of luteolin for 24 h. Protein extracts were isolated and subjected to Western blot analysis with indicated antibodies. One of four independent experiments is shown (a). 786-O cells were pretreated with medium, SB203580, SP600125, U0126, or insulin for 1 h and then were treated with luteolin for additional 24 h. Cell viability was determined by MTS reduction assay (b). Protein extracts were isolated and subjected to fluorogenic caspase-3 assay. The intensity of fluorescent signals was expressed as arbitrary unit (c). ** versus medium control and ## versus luteolin control, .
