Induction of Apoptosis by Luteolin Involving Akt Inactivation in Human 786-O Renal Cell Carcinoma Cells

Figure 6

Effects of agents on cell viability. 786-O cells were treated with medium, luteolin, 17-AAG, or LY294002 alone, or in combinations for 24 h. Cell viability was determined by MTS reduction assay (a). Protein extracts were isolated and subjected to fluorogenic caspase-3 assay. The intensity of fluorescent signals was expressed as arbitrary unit (b). **𝑃<0.01 versus medium control, ^##𝑃<0.01 versus luteolin control, ^&&𝑃<0.01 versus luteolin/17-AAG, and ^%%𝑃<0.01 versus luteolin/LY294002, 𝑛=4.

(a)

(b)