Fermented Wheat Germ Extract Induced Cell Death and Enhanced Cytotoxicity of Cisplatin and 5-Fluorouracil on Human Hepatocellular Carcinoma Cells
Figure 1
FWGE treatment inhibited cell growth of human HCC cells, HepG2, 3B, and J5. (a) to (c) cells were treated with a serial dilutions of FWGE in HepG2, 3B, and J5 cells for 48 or 72 hr. Cell viability was analyzed by MTT assay. Experiments were performed in triplicate at least and data were shown as mean ± SD. All cell lines showed dose-dependent effects at 72 hr after FWGE treatment in the MTT assay (one-way ANOVA, ). (d) to (f) where FWGE-induced morphological changes in HepG2, Hep3B, and HepJ5 cells treated with 0.4, 0.25 and 1 mg/mL for 72 hr. Arrows indicated morphological changes in FWGE treated cells. (g) Western blotting analysis of PARP for Hep3B cells treated with 0.25 mg/mL FWGE for 72 hr. PARP was 116 KDa and cleaved PARP was 89 kDa.
