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Figure 2: The effects of baicalein on the mitochondrial membrane potential and cytochrome c release in CCRF-CEM cells. (a) The cells were stained with JC-1 fluorescence dye, followed by flow cytometry analysis. The data are representative of three independent experiments with similar results. Means ± SD of the experimental replicates is presented with bar graph in the bottom. * , compared with the untreated control group. (b) The cytosolic fraction of lysates was subjected to SDS-PAGE, followed by Western blot analysis with the anti-cytochrome c antibody. Bands were analyzed by ImageJ and normalized to actin. The data are representative of three independent experiments showing similar results. Means ± SD of the three independent experiments is presented with bar graph in the bottom. * , compared with the untreated control group. ** , compared with the untreated control group.