124747.fig.009a
124747.fig.009b
124747.fig.009c
Figure 9: The combined therapeutic efficacy of baicalein and vincristine in CCRF-CEM cells. (a) 4 × 104 cells/well were seeded onto 24-well culture plates, followed by incubation with the indicated doses of baicalein, vincristine or both for 24 h. The cell viability was then determined by the MTT assay. The data are presented as the mean ± SD from triplicate wells. Similar results were obtained in three independent experiments. ** , compared with the group treated with vincristine alone. * , compared with the group treated with baicalein alone. (b) Cells were seeded at 3 × 105 cells/dish onto 6-cm culture dishes and incubated with the indicated doses of baicalein and/or vincristine for 24 h. After staining with annexin V-FITC and propidium iodide (PI), the cells were analyzed by flow cytometry. The data are representative of two independent experiments showing similar results. (c) Cells were treated with the indicated concentrations of baicalein for 8 or 24 h, after which the cell cycle analysis was determined by propidium iodide (PI) staining and subsequent flow cytometry analysis. The data are representative of two independent experiments showing similar results.