Molecular Mechanisms Underlying the In Vitro Anti-Inflammatory Effects of a Flavonoid-Rich Ethanol Extract from Chinese Propolis (Poplar Type)
Figure 4
Effects of EECP on the phosphorylation of IκB-α and AP-1 in LPS-stimulated RAW 264.7 cells. (a) RAW 264.7 cells were stimulated with LPS (1 μg/mL) for indicated times or (b) were pretreated with EECP (15 μg/mL) for 1 h then stimulated with LPS (1 μg/mL) for indicated times. (c) RAW 264.7 cells were pretreated or not with indicated concentrations of EECP for 1 h then were stimulated with LPS (1 μg/mL) for 30 min. Whole cell lysates were analyzed by Western blotting analysis using specific antibodies. The relative expression of proteins was quantified using Quantity One software comparing with β-tubulin. Data shown are the representative of three independent experiments with similar results.
