Table 1: Counting of daily detached, trypan blue stained and viable LLC-PK1 cells during the GM exposure and SFN treatment.

CTSFNGMSFN + GM

DC/well 24 h1,483 ± 367483 ± 605,487 ± 247*1,113 ± 567#
DC/well 48 h1,633 ± 1091,117 ± 4698,033 ± 324*4,250 ± 375#
DC/well 72 h2,867 ± 1922,883 ± 2598,017 ± 760ε4,733 ± 838Δ
TBS cells/well (attached after GM exposure)16,517 ± 3,21923,733 ± 8,02031,800 ± 3,31825,433 ± 829
Total death cells/well22,500 ± 3,39928217 ± 8,36653,337 ± 3,714ε35,550 ± 1,422
Viable cells/well106,450 ± 4,97694,317 ± 5,87426,883 ± 4,681*60,400 ± 5,765Δ

CT: cells without treatment. SFN: cells incubated in 5 µM SFN. GM: cells in 8 mM GM. SFN + GM: cells coincubated in 5 µM SFN and 8 mM GM. DC: detached cells. TBS: trypan blue stained. Total death cells = DC 24 h + DC 48 h + DC 72 h + TBS cells. Cells attached after GM exposure include TBS cells + viable cells. Data are mean ± SEM, . versus CT; versus GM; versus CT; versus GM.