Electroacupuncture Ameliorates Learning and Memory via Activation of the CREB Signaling Pathway in the Hippocampus to Attenuate Apoptosis after Cerebral Hypoperfusion

Figure 3

The traces of the 60 s spatial probe trials, the frequency of swimming across the platform site, and the swimming speed of the five groups. Cerebral hypoperfusion effected the spatial probe trial as demonstrated by the decreased frequency of swimming across the platform during the 60 s interval. In the EA group, the frequency increased (a); after ICV injection of H89, the increased frequency decreased (b), while there were no significant differences in the swimming speed either among the control, model, and EA groups or between the EA + NS and EA + H89 groups ((c) and (d)). The data are expressed as the mean ± SEM. ANOVA statistical analyses were performed to compare the means among the control, model, and EA groups; ^※𝑃<0.05 compared with the control group; ^▲𝑃<0.05 compared with the model group. A 𝑡-test was used to compare the means between the EA + NS and EA + H89 groups; *𝑃<0.05 compared with the EA + NS group.