Electroacupuncture Ameliorates Learning and Memory via Activation of the CREB Signaling Pathway in the Hippocampus to Attenuate Apoptosis after Cerebral Hypoperfusion

Figure 4

Western blotting showing immunoreactivity of phosphorylated CREB in the hippocampal CA1 area. The pCREB protein values were calculated as a ratio of pCREB protein to GAPDH. EA increased the expression of pCREB protein (a), and the EA-induced activation of pCREB was markedly inhibited by ICV injection of H89 (b). The data are expressed as the mean ± SEM. ANOVA statistical analyses were performed to compare the means among the control, model, and EA groups; ^※𝑃<0.05 compared with the control group; ^▲𝑃<0.05 compared with the model group. A 𝑡-test was used to compare the means between the EA + NS and EA + H89 groups; *𝑃<0.05 compared with the EA + NS group.