Evaluation of the Bronchorelaxant, Genotoxic, and Antigenotoxic Effects of Cassia alata L.

Table 4

EC₅₀ values and potency (pEC₅₀) of CF-AECal-induced relaxation of rat tracheal smooth muscle contracted by 10 µM acetylcholine under various experimental conditions.

Treatment groups	EC50 (mg/mL)	pEC50
Control	0.021 (0.013–0.031)	1.68 ± 0.08
L-NAME (100 µM)	0.018 (0.010–0.031)	1.4 ± 0.09
Indomethacin (10 µM)	0.019 (0.011–0.031)	1.73 ± 0.11
Methylene blue (100 µM)	0.021 (0.008–0.054)	1.68 ± 0.15
Propranolol (1 µM)	0.029 (0.010–0.081)	1.54 ± 0.16
TEA (5 mM)	0.184 (0.123–0.274)	0.74 ± 0.08***
Glibenclamide (10 µM)	0.022 (0.015–0.032)	1.65 ± 0.07

EC₅₀ was obtained from the concentration-response curve of CF-AECal. Data are expressed as the mean ± SEM of 6–8 experiments. Significantly different from control: *𝑃 < 0.05, **𝑃 < 0.01, and ***𝑃 < 0.001.