Populus balsamifera Extract and Its Active Component Salicortin Reduce Obesity and Attenuate Insulin Resistance in a Diet-Induced Obese Mouse Model

Table 1

Effects of obesity, P. balsamifera, and salicortin treatments on body and organ weights at sacrifice.

	DIO	P. balsamifera 125 mg/kg	P. balsamifera 250 mg/kg	DIO	Salicortin; 12.5 mg/kg
Body Weight	138 ± 1^†	120 ± 7*	131 ± 3	142 ± 2^†	130±3^§
Retroperitoneal Fat Pad	229 ± 12^†	190 ± 28	209 ± 20	242 ± 13^†	218 ± 10
Epididymal Fat Pad	77 ± 3^†	98 ± 17	103 ± 5*	97 ± 2	149±11^§
Brown Adipose tissue	189 ± 14^†	136 ± 22; 𝑃=0.057	168 ± 14	200 ± 6^†	170±11^§
Liver Weight	167 ± 6^†	108 ± 12*	122 ± 10*	166 ± 9^†	118±7^§
Total Kidney	111 ± 4^†	93 ± 3*	104 ± 4	104 ± 2	101 ± 3

Measurements were obtained after 16 weeks of treatment with either standard diet (Chow), HFD (DIO), and for the last 8 of the 16 weeks with HFD in combination with P. balsamifera at 125 or 250 mg/kg, or with the active salicortin at 12.5 mg/kg. All values are expressed as a percentage of respective Chow controls (reference set at 100%) and represent the mean ± SEM. The number of animals for each group for the P. balsamifera protocol was: CHOW (𝑛=12); DIO (𝑛=8); P. balsamifera 125 (𝑛=5); P. balsamifera 250 (𝑛=7); and for the salicortin protocol: CHOW (𝑛=12); DIO (𝑛=7); salicortin (𝑛=9). ^†denotes DIO significantly different as compared to Chow (unpaired Student’s t test; P < 0.05). *denotes significantly different as compared to respective DIO (one way ANOVA, Bonferroni post hoc test; P < 0.05). §denotes significantly different as compared to respective DIO (unpaired Student’s t test; P < 0.05).